Nisin Augments Doxorubicin Permeabilization and Ameliorates Signaling Cascade during Skin Carcinogenesis
نویسندگان
چکیده
All the chemicals used in the present study were of analytical grade and were obtained from standard companies. ELISA plates, tissue culture plates (96 /8 well), culture dishes, Serological plates, Roux culture bottles, 0.2μm sterile syringe filters (non-pyrogenic). DMBA (7,12-Dimethylbenzylanhracene), TPA (12-O-tetradecanoylphorbol13-acetate), RPMI 1640 (Rose Well Park Memorial Institute Lab no. 1640) (Himedia, India), doxorubicin nisinand3-(4,5-dimethylthiazol2yl)-2,5-diphenyl tetrazolium bromide were procured from Sigma Aldrich.N-phenyl-1-napthylamine (NPN), bovine serum albumin (BSA), 5,5-dithiobis-2-nitrobenzoic acid (DTNB), thiobarbituric acid (TBA), ethanol, formaldehyde, hydrogen peroxide (H2O2), benzene, paraffin wax, xylene, trichloroacetic acid (TCA), sodium chloride (NaCl), EDTA, Folin’s Reagent, sodium carbonate, copper sulphate, Abstract
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